Composite

Part:BBa_K1615070:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-28)


TVEL5 fused to CBDcenA+Linker in RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
    Illegal BglII site found at 773
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418


Design Notes

Fusion done using RFC25.



Source

TVEL5 Laccase from Tramates versicolor. CBDcenA from Cellulomonas fimi.

References